Bethyl Laboratories
Rabbit anti-HMGN3 Antibody Affinity Purified
Primary
Catalog #A301-192A
TargetHMGN3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
HMGN3 was originally identified in a screen for interaction partners of the thyroid hormone receptor. HMGN3 belongs to the HMGN family of small basic proteins that interacts specifically with nucleosomes and functions to facilitate chromatin unfolding to allow access of transcription factors to DNA targets. HMGN has been found to be a regulator of the glycine transporter 1 (GLYT1) gene.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 49 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetHMGN3
Antigen Species
Human
Gene ID9324
Gene Symbol
HMGN3
Gene Name
high mobility group nucleosomal binding domain 3
Uniprot IDQ15651
Protein Name
High mobility group nucleosome-binding domain-containing protein 3
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to HMGN3 immobilized on solid support.
The epitope recognized by A301-192A maps to a region between residue 60 and 99 of human high mobility group nucleosomal binding domain 3 using the numbering given in entry NP_004233.1 (GeneID 9324).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-192A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-192A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-192AOpen
Services
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