Bethyl Laboratories
Rabbit anti-hnRNP-L Antibody Affinity Purified
Primary
Catalog #A303-896A
TargethnRNP-L
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
Image 1 of 5
local_shipping
Order within 15 hours and 34 minutes for shipment by today
Product Description
hnRNP-L is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HnRNP-L Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. It associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter cytoplasm [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P14866].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 539 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargethnRNP-L
Antigen Species
Human
Gene ID3191
Gene Symbol
HNRNPL
Gene Name
heterogeneous nuclear ribonucleoprotein L
Uniprot IDP14866
Protein Name
Heterogeneous nuclear ribonucleoprotein L
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to hnRNP-L immobilized on solid support.
The epitope recognized by A303-896A maps to a region between residue 539 and 589 of human Heterogeneous Nuclear Ribonucleoprotein L using the numbering given in entry NP_001524.2 (GeneID 3191).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-896A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-896A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-896AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1