Bethyl Laboratories

Rabbit anti-HPIP IHC Antibody Affinity Purified

Primary
Catalog #IHC-00327
TargetHPIP
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF
Detection of human HPIP by immunohistochemistry.
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Product Description

Hematopoietic PBX-interacting protein (HPIP) was identified in a yeast two-hybrid screen for factors that interact with PBX1, a homeodomain protein that regulates gene transcription during development and differentiation. Recently, HPIP has been reported to interact with estrogen receptor alpha (ERalpha) and estrogen receptor beta (ERbeta) and modulate ER target gene expression.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetHPIP
Antigen Species
Human
Gene ID57326
Gene Symbol
PBXIP1
Gene Name
PBX homeobox interacting protein 1
Uniprot IDQ96AQ6
Protein Name
Pre-B-cell leukemia transcription factor-interacting protein 1
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to HPIP immobilized on solid support.

The epitope recognized by IHC-00327 maps to a region between residue 1 and 50 of human hematopoietic PBX-interacting protein using the numbering given in entry NP_065385.2 (GeneID 57326).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Epitope exposure is recommended.

Epitope exposure with citrate buffer will enhance staining.

Likely to work with frozen sections.

In some cases, the antibody may be diluted further than indicated.

Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500

Documents

Safety Data SheetPDF · IHC-00327Open
Product DatasheetPDF · IHC-00327 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00327 · Lot 1 (current lot)Open
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