Bethyl Laboratories
Rabbit anti-IARS Antibody Affinity Purified
Primary
Catalog #A304-747A
TargetIARS
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase (IARS) belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis [taken from NCBI Entrez Gene (Gene ID: 3376)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetIARS
Antigen Species
Human
Gene ID3376
Gene Symbol
IARS
Gene Name
isoleucyl-tRNA synthetase
Uniprot IDP41252
Protein Name
Isoleucine--tRNA ligase, cytoplasmic
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to IARS immobilized on solid support.
The epitope recognized by A304-747A maps to a region between residue 1 to 50 of human Isoleucyl-tRNA Synthetase using the numbering given in entry NP_002152.2 (GeneID 3376).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-747A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-747A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-747AOpen
Citations / Publications
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