Bethyl Laboratories

Rabbit anti-ICAM-1 Antibody Affinity Purified

Primary
Catalog #A302-873A
TargetICAM-1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
Detection of human ICAM-1 by western blot and immunoprecipitation.
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Product Description

Intercellular adhesion molecule 1 (ICAM-1) is a ligand for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM-1 is also the cellular receptor for rhinovirus and coxsackievirus.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetICAM-1
Antigen Species
Human
Gene ID3383
Gene Symbol
ICAM1
Gene Name
intercellular adhesion molecule 1
Uniprot IDP05362
Protein Name
Intercellular adhesion molecule 1
Product Information
Applications
WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to ICAM-1 immobilized on solid support.

The epitope recognized by A302-873A maps to a region between residue 1 and 50 of human Intercellular Adhesion Molecule 1 using the numbering given in entry NP_000192.2 (GeneID 3383).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:2000 - 1:10,000

Documents

Product DatasheetPDF · A302-873A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-873A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-873AOpen
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