Bethyl Laboratories
Rabbit anti-IDE Antibody Affinity Purified
Primary
Catalog #A301-906A
TargetIDE
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationWB
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Product Description
Insulin-degrading enzyme (IDE) is a metalloendopeptidase that plays a primary role in the regulated degradation of insulin. In the cell, IDE is found mostly in the soluble cytosolic fraction, and small amounts exist in the plasma membrane, endosomes, and peroxisomes; however the highest relative concentration is found in the peroxisomes. IDE is proposed to be a multifunctional enzyme since it is also present in non-insulin sensitive cells. Other substrates of IDE include glucagon, IGF-II, atrial natriuretic factor, and TGF-alpha.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 275 and 325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetIDE
Antigen Species
Human
Gene ID3416
Gene Symbol
IDE
Gene Name
insulin degrading enzyme
Uniprot IDP14735
Protein Name
Insulin-degrading enzyme
Alternate Names
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to IDE immobilized on solid support.
The epitope recognized by A301-906A maps to a region between residue 275 and 326 of human insulin-degrading enzyme using the numbering given in entry NP_004960.1 (GeneID 3416).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-906A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-906A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-906AOpen
Services
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