/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-IDH2 Antibody
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Primary Antibodies  

Rabbit anti-IDH2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (2)
Target:

IDH2

Reactivity:

Human

,

Mouse

Applications:

ICC-IF

,

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 402 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Presumed Reactivity

Pig

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to IDH2 immobilized on solid support.

The epitope recognized by A304-096A maps to a region between residue 402 to 452 of human Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial using the numbering given in entry NP_002159.2 (GeneID 3418).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to IDH2 immobilized on solid support.

The epitope recognized by A304-096A-T maps to a region between residue 402 to 452 of human Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial using the numbering given in entry NP_002159.2 (GeneID 3418).

Country of Origin

USA

Additional Product Information

Isocitrate dehydrogenase 2 (NADP+), mitochondrial (IDH2) plays a role in intermediary metabolism and energy production. IDH2 may tightly associate or interact with the pyruvate dehydrogenase complex [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P48735].

3418

IDH2

isocitrate dehydrogenase (NADP(+)) 2, mitochondrial

P48735

Isocitrate dehydrogenase [NADP], mitochondrial

Alternate Names

D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; isocitrate dehydrogenase NADP, mitochondrial; isocitrate dehydrogenase 2 (NADP+), mitochondrial; mNADP-IDH; NADP(+)-specific ICDH; oxalosuccinate decarboxylase

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.