Bethyl Laboratories
Rabbit anti-IGF2BP2 Antibody Affinity Purified
Primary
Catalog #A303-316A
TargetIGF2BP2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific and may regulate translation of target mRNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q9Y6M1].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetIGF2BP2
Antigen Species
Human
Gene ID10644
Gene Symbol
IGF2BP2
Gene Name
insulin like growth factor 2 mRNA binding protein 2
Uniprot IDQ9Y6M1
Protein Name
Insulin-like growth factor 2 mRNA-binding protein 2
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to IGF2BP2 immobilized on solid support.
The epitope recognized by A303-316A maps to a region between residue 150 and 200 of human Insulin-Like Growth Factor 2 mRNA Binding Protein 2 using the numbering given in entry NP_006539.3 (GeneID 10644).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-316A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-316A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-316AOpen
Citations / Publications
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