Bethyl Laboratories

Rabbit anti-IL-13 Recombinant Monoclonal Antibody [BLR329M]

Primary
Catalog #A700-329
TargetIL-13
HostRabbit
CloneBLR329M
ReactivityMouse, Human
ApplicationIP, WB
Detection of rat and human IL-13 by western blot.
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Product Description

IL-13 is a cytokine that plays important roles in allergic inflammation and in the immune response to parasite infection. IL-13 exerts its biological effects through its receptors which comprises the IL4R chain and the IL13RA1 chain, which activate the JAK/STAT pathway. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P35225].

Specifications

Clonality
Recombinant Monoclonal
Clone
BLR329M
Host
Rabbit
Immunogen
residues 35-146
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetIL-13
Antigen Species
Human
Gene ID3596
Gene Symbol
IL13
Gene Name
interleukin 13
Uniprot IDP35225
Protein Name
Interleukin-13
Alternate Names
IL-13  •  interleukin-13  •  P600
Product Information
Applications
IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Recombinant antibody was purified from cell culture supernatant.

Immunogen was a recombinant protein representing human Interleukin 13 (residues 35-146, NP_002179.2).

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Western Blot (WB)1:1,000
Immunoprecipitation (IP)6 µl/mg lysate

Documents

Product DatasheetPDF · A700-329 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-329 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-329Open
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