Bethyl Laboratories
Rabbit anti-ILK IHC Antibody Affinity Purified
Primary
Catalog #IHC-00361
TargetILK
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF
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Product Description
Integrin-linked kinase (ILK) is a serine/threonine kinase associated with the cytoplasmic domain of beta integrins and mediates integrin signaling from the extracellular matrix to the intracellular actin cytoskeleton. PKB/Akt and GSK-3beta are targets of ILK involved in integrin-mediated cell proliferation and cell survival. ILK has been shown to interact with Paxillin, PINCH-1, PINCH-2, and parvin to influence actin dynamics.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 402 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetILK
Antigen Species
Human
Gene ID3611
Gene Symbol
ILK
Gene Name
integrin linked kinase
Uniprot IDQ13418
Protein Name
Integrin-linked protein kinase
Alternate Names
Product Information
Applications
ICC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ILK immobilized on solid support.
The epitope recognized by IHC-00361 maps to a region between residue 402 and 451 of human integrin-linked kinase using the numbering given in entry NP_004508.1 (GeneID 3611).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Acetone-Fixed Cells 1:100 - 1:500:
Formaldehyde fixation is not recommended.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00361Open
Product DatasheetPDF · IHC-00361 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00361 · Lot 1 (current lot)Open
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