Bethyl Laboratories
Rabbit anti-Importin 7 Antibody Affinity Purified
Primary
Catalog #A302-727A
TargetImportin 7
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Importin 7 autonomously mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. It binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 it mediates the nuclear import of H1 histone. In vitro, importin 7 mediates nuclear import of H2A, H2B, H3 and H4 histones. Also, in vitro, it mediates the nuclear import of HIV-1 reverse transcription complex (RTC) integrase and HIF-1 alpha [taken from the Universal Protein Resource (UniProt) ww.uniprot.org/uniprot/O95373].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetImportin 7
Antigen Species
Human
Gene ID10527
Gene Symbol
IPO7
Gene Name
importin 7
Uniprot IDO95373
Protein Name
Importin-7
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Importin 7 immobilized on solid support.
The epitope recognized by A302-727A maps to a region between residue 200 and 250 of human Importin 7 using the numbering given in entry NP_006382.1 (GeneID 10527).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-727A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-727A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-727AOpen
Citations / Publications
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