Bethyl Laboratories

Rabbit anti-INA Antibody Affinity Purified

Primary
Catalog #A305-431A
TargetINA
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Detection of human INA by western blot of immunoprecipitates.
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Product Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites.INA is a member of the intermediate filament family and is involved in the morphogenesis of neurons [taken from NCBI Entrez Gene (Gene ID: 9118)].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 300 and 350
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetINA
Antigen Species
Human
Gene ID9118
Gene Symbol
INA
Gene Name
internexin neuronal intermediate filament protein alpha
Uniprot IDQ16352
Protein Name
Alpha-internexin
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to INA immobilized on solid support.

The epitope recognized by A305-431A maps to a region between residue 300 to 350 of human Alpha-internexin using the numbering given in entry Q16352.2 (GeneID 9118).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A305-431A · Lot 1 (current lot)Open
Product DatasheetPDF · A305-431A · Lot 1 (current lot)Open
Safety Data SheetPDF · A305-431AOpen
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