Bethyl Laboratories
Rabbit anti-INT1 Antibody Affinity Purified
Primary
Catalog #A300-361A
TargetINT1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
INT1 was first identified as KIAA1440, a hypothetical protein predicted from the in silico analysis of long cDNAs isolated in the Kazusa cDNA sequencing project. Targeted disruption of INT1 results in growth arrest at the early blastocyst stage, activated caspase-3/7 that results in apoptosis, and impaired processing of U2 snRNA. INT1 has been demonstrated to be the largest subunit of the integrator complex which functions to mediate U1 and U2 snRNA 3’end processing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 25 and 75
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetINT1
Antigen Species
Human
Gene ID26173
Gene Symbol
INTS1
Gene Name
integrator complex subunit 1
Uniprot IDQ8N201
Protein Name
Integrator complex subunit 1
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to INT1 immobilized on solid support.
The epitope recognized by A300-361A maps to a region between residues 150 and 200 of human integrator complex subunit 1 using the numbering given in entry NP_001073922.1 (GeneID 26173).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:2500 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A300-361A · Lot 2 (current lot)Open
Product DatasheetPDF · A300-361A · Lot 2 (current lot)Open
Safety Data SheetPDF · A300-361AOpen
Citations / Publications
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