/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-INT11 Antibody
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Primary Antibodies  

Rabbit anti-INT11 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (21)
Target:

INT11

Reactivity:

Human

,

Mouse

Applications:

IHC

,

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 550 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to INT11 immobilized on solid support.

The epitope recognized by A301-274A maps to a region between residue 550 and 600 of human integrator complex subunit 11 using the numbering given in entry NP_060341.2 (GeneID 54973).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

INT11 has been identified as a component of the twelve-subunit integrator complex that functions in RNA Pol II mediated 3’-end mRNA processing of the U1 and U2 small nuclear RNAs (snRNAs). INT11 is a beta-lactamase family member that has sequence similarity to the cleavage and polyadenylation specificity factors CPSF-73 and CPSF-100.

54973

INTS11

integrator complex subunit 11

Q5TA45

Integrator complex subunit 11

Alternate Names

cleavage and polyadenylation specific factor 3-like; cleavage and polyadenylation-specific factor 3-like protein; CPSF3L; CPSF3-like protein; CPSF73L; INT11; integrator complex subunit 11; protein related to CPSF subunits of 68 kDa; RC68; RC-68; related to CPSF subunits 68 kDa

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.