Bethyl Laboratories
Rabbit anti-INT7 Antibody Affinity Purified
Primary
Catalog #A300-271A
TargetINT7
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
INT7 is subunit 7 of the integrator complex. The integrator complex is composed of at least 12 subunits and associates with the C-terminal domain of RNA polymerase II to mediate 3’-end processing of small-nuclear RNAs (snRNAs).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 850 and 900
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetINT7
Antigen Species
Human
Gene ID25896
Gene Symbol
INTS7
Gene Name
integrator complex subunit 7
Uniprot IDQ9NVH2
Protein Name
Integrator complex subunit 7
Alternate Names
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific INT7 to immobilized on solid support.
The epitope recognized by A300-271A maps to a region between residues 850 and 900 of human INT7 using the numbering given in TrEMBL entry Q9NVH2 (GeneID 25896).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A300-271A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-271A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-271AOpen
Citations / Publications
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Resources
Standard Western Blot Protocol
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