Rabbit anti-Integrin Alpha 6 Antibody Affinity Purified

Product Details
Specifications
The epitope recognized by A303-681A-T maps to a region between residue 50 and 100 of human Integrin, Alpha 6 using the numbering given in entry EAX11175.1 (GeneID 3655).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4. Antibody was affinity purified using an epitope specific to Integrin Alpha 6 immobilized on solid support.
The epitope recognized by A303-681A maps to a region between residue 50 and 100 of human Integrin, Alpha 6 using the numbering given in entry EAX11175.1 (GeneID 3655).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Additional Product Information
Integrin alpha 6 forms heterodimers with integrin beta 1 or beta 4 and functions as a receptor for the extracellular matrix protein, laminin. Integrin alpha 6 is encoded by the ITGA6 gene. Defects in ITGA6 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P23229].
Alternate Names
CD antigen CD49f; CD49 antigen-like family member F; CD49f; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6B; VLA-6
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.