Bethyl Laboratories
Rabbit anti-IP3R1 Antibody Affinity Purified
Primary
Catalog #A302-158A
TargetIP3R1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IHC-IF, IP, WB
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Product Description
IP3R1 (inositol 1,4,5-triphosphate receptor type 1) is an intracellular calcium release channel that mediates calcium release from the endoplasmic reticulum. There are 3 mammalian InsP3R receptors: Insp3R1, Insp3R2, and Insp3R3. Defects in the gene which encodes InsP3R1, ITPR1, are the cause of spinocerebellar ataxia type 15 (SCA15).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2708 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetIP3R1
Antigen Species
Human
Gene ID3708
Gene Symbol
ITPR1
Gene Name
inositol 1,4,5-trisphosphate receptor type 1
Uniprot IDQ14643
Protein Name
Inositol 1,4,5-trisphosphate receptor type 1
Alternate Names
ACV • CLA4 • inositol 1,4,5-triphosphate receptor, type 1 • inositol 1,4,5-trisphosphate receptor type 1 • INSP3R1 • IP3 receptor • IP3 receptor isoform 1 • IP3R • IP3R 1 • IP3R1 • PPP1R94 • protein phosphatase 1, regulatory subunit 94 • SCA15 • SCA16 • SCA29 • type 1 inositol 1,4,5-trisphosphate receptor • type 1 InsP3 receptor
Product Information
Applications
IHC, IHC-IF, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to IP3R1 immobilized on solid support.
The epitope recognized by A302-158A maps to a region between residue 2708 and 2758 of human inositol 1, 4,5-trisphosphate receptor type 1 using the numbering given in entry Q14643.2 (GeneID 3708).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:250
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:1000 - 1:5000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A302-158A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-158A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-158AOpen
Citations / Publications
Services
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