Bethyl Laboratories
Rabbit anti-IQGAP1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00399
TargetIQGAP1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
IQGAP1 (IQ motif containing GTPase activating protein 1) interacts with the Rho-family GTPases, CDC42 and RAC1. It has been shown to localize to sites of cell-cell contact and regulate cadherin-mediated cell-cell adhesion. In addition to its role in cell adhesion, it is also involved in cell polarization and migration.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetIQGAP1
Antigen Species
Human
Gene ID8826
Gene Symbol
IQGAP1
Gene Name
IQ motif containing GTPase activating protein 1
Uniprot IDP46940
Protein Name
Ras GTPase-activating-like protein IQGAP1
Alternate Names
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to IQGAP1 immobilized on solid support.
The epitope recognized by IHC-00399 maps to a region between residue 1 and 50 of human motif containing GTPase activating protein 1 using the numbering given in entry NP_003861.1 (GeneID 8826).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate or tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00399Open
Product DatasheetPDF · IHC-00399 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00399 · Lot 1 (current lot)Open
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