/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-IRS2 Antibody
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Primary Antibodies  

Rabbit anti-IRS2 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

IRS2

Reactivity:

Human

Applications:

IHC

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1288 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to IRS2 immobilized on solid support.

The epitope recognized by A301-434A-T maps to a region between residue 1288 and 1338 of human insulin receptor substrate 2 using the numbering given in entry NP_003740.2 (GeneID 8660).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to IRS2 immobilized on solid support.

The epitope recognized by A301-434A maps to a region between residue 1288 and 1338 of human insulin receptor substrate 2 using the numbering given in entry NP_003740.2 (GeneID 8660).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

IRS2 (insulin receptor substrate 2) functions as a cytoplasmic link and transducer of insulin receptor (IR) and insulin-like growth factor receptor (IGFR) signaling. As the name implies, IRS2 is a substrate of IR and IGFR tyrosine kinase activity activated by insulin and IGF binding.

8660

IRS2

insulin receptor substrate 2

Q9Y4H2

Insulin receptor substrate 2

Alternate Names

insulin receptor substrate 2; IRS-2

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.