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Rabbit anti-IWS1 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
IWS1
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

100 µl (10 blots)

20 µl (2 blots)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Mouse, Human
Presumed Reactivity
Rat
Antigen Species
Human
Stock Concentration
400 µg/ml
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to IWS1 immobilized on solid support.

The epitope recognized by A304-609A-T maps to a region between residue 1 to 50 of human IWS1-Like Protein using the numbering given in entry NP_060439.2 (GeneID 55677).

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Gene & Protein Information

IWS1 homolog is a transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. IWS1 acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96ST2].

55677

IWS1

IWS1, SUPT6H interacting protein

Q96ST2

Protein IWS1 homolog

Alternate Names

interacts with Spt6, IWS1 homolog, IWS1-like protein, protein IWS1 homolog

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Citations (1)