Bethyl Laboratories
Rabbit anti-JAM-A Antibody Affinity Purified
Primary
Catalog #A302-891A
TargetJAM-A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
Junctional adhesion molecule A (JAM-A) is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor [taken from NCBI Entrez Gene (Gene ID: 50848)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 249 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetJAM-A
Antigen Species
Human
Gene ID50848
Gene Symbol
F11R
Gene Name
F11 receptor
Uniprot IDQ9Y624
Protein Name
Junctional adhesion molecule A
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to JAM-A immobilized on solid support.
The epitope recognized by A302-891A maps to a region between residue 249 and 299 of human Junctional Adhesion Molecule A using the numbering given in entry NP_058642.1 (GeneID 50848).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-891A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-891A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-891AOpen
Citations / Publications
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