Bethyl Laboratories
Rabbit anti-JARID1B IHC Antibody Affinity Purified
Primary
Catalog #IHC-00370
TargetJARID1B
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
JARID1B (Jumonji AT-rich interactive domain 1B) is a member of the JARID 1 family of proteins that catalyze the demethylation of histones on lysine. The Jarid 1 family members include JARID1A, JARID1B, JARID1C and JARID1D. The members of this family possess a Jumonji C (JmjC) and Jumonji N (JmjN) domain as well as one ARID (AT-rich interactive domain) and three PHD-type zinc fingers. As histone demethylases, JARID 1 proteins function as transcriptional repressors. JARID1B expression is largely restricted to testis and may play a critical role in tumorigenesis as it has been found to be upregulated in prostate and breast cancer.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1494 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetJARID1B
Antigen Species
Human
Gene ID10765
Gene Symbol
KDM5B
Gene Name
lysine demethylase 5B
Uniprot IDQ9UGL1
Protein Name
Lysine-specific demethylase 5B
Alternate Names
cancer/testis antigen 31 • CT31 • histone demethylase JARID1B • JARID1B • jumonji, AT rich interactive domain 1B • jumonji/ARID domain-containing protein 1B • lysine (K)-specific demethylase 5B • lysine-specific demethylase 5B • MRT65 • PLU1 • PLU-1 • PPP1R98 • protein phosphatase 1, regulatory subunit 98 • PUT1 • putative DNA/chromatin binding motif • RBBP2H1A • RBP2-H1 • retinoblastoma-binding protein 2 homolog 1 • retinoblastoma-binding protein 2, homolog 1A
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to JARID1B immobilized on solid support.
The epitope recognized by IHC-00370 maps to a region between residue 1494 and 1544 of human Jumonji, AT rich interactive domain 1B using the numbering given in entry NP_006609.3 (GeneID 10765).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00370Open
Product DatasheetPDF · IHC-00370 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00370 · Lot 1 (current lot)Open
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