Bethyl Laboratories
Rabbit anti-JARID1C Antibody Affinity Purified
Primary
Catalog #A301-035A
TargetJARID1C
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
JARID1C is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. Some recently characterized members of the jmj family include JARID1A/RBP2, JARID1C, JMJD1A, JMJD1B, JMJD1C, JMJD2A, JMJD2C and JMJD2D. Mutations in JARID1C are associated with X-linked mental retardation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1510 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetJARID1C
Antigen Species
Human
Gene ID8242
Gene Symbol
KDM5C
Gene Name
lysine demethylase 5C
Uniprot IDP41229
Protein Name
Lysine-specific demethylase 5C
Alternate Names
DXS1272E • histone demethylase JARID1C • JARID1C • JmjC domain-containing protein SMCX • Jumonji, AT rich interactive domain 1C (RBP2-like) • Jumonji/ARID domain-containing protein 1C • lysine (K)-specific demethylase 5C • lysine-specific demethylase 5C • MRX13 • MRXJ • MRXSCJ • MRXSJ • Protein SmcX • Protein Xe169 • SMCX • Smcx homolog, X chromosome • Smcy homolog, X-linked • XE169
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to JARID1C immobilized on solid support.
The epitope recognized by A301-035A maps to a region between residue 1510 and the C-terminus (residue 1560) of human Jumonji, AT rich interactive domain 1C using the numbering given in entry NP_004178.2 (GeneID 8242).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-035A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-035A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-035AOpen
Citations / Publications
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