/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-JMJD2B Antibody
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Primary Antibodies  

Rabbit anti-JMJD2B Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (4)
Target:

JMJD2B

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 625 and 675

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to JMJD2B immobilized on solid support.

The epitope recognized by A301-477A-T maps to a region between residue 625 and 675 of human jumonji domain containing 2B using the numbering given in entry NP_055830.1 (GeneID 23030).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to JMJD2B immobilized on solid support.

The epitope recognized by A301-477A maps to a region between residue 625 and 675 of human jumonji domain containing 2B using the numbering given in entry NP_055830.1 (GeneID 23030).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

JMJD2B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JMJD2B has been shown to be capable of antagonizing H3K9 trimethylation.

23030

KDM4B

lysine demethylase 4B

O94953

Lysine-specific demethylase 4B

Alternate Names

jmjC domain-containing histone demethylation protein 3B; JMJD2B; jumonji domain containing 2B; jumonji domain-containing protein 2B; lysine (K)-specific demethylase 4B; lysine-specific demethylase 4B; TDRD14B; tudor domain containing 14B

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.