Bethyl Laboratories
Rabbit anti-JMJD2B Antibody Affinity Purified
Primary
Catalog #A301-477A
TargetJMJD2B
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
JMJD2B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JMJD2B has been shown to be capable of antagonizing H3K9 trimethylation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 625 and 675
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetJMJD2B
Antigen Species
Human
Gene ID23030
Gene Symbol
KDM4B
Gene Name
lysine demethylase 4B
Uniprot IDO94953
Protein Name
Lysine-specific demethylase 4B
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to JMJD2B immobilized on solid support.
The epitope recognized by A301-477A maps to a region between residue 625 and 675 of human jumonji domain containing 2B using the numbering given in entry NP_055830.1 (GeneID 23030).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-477A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-477A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-477AOpen
Citations / Publications
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