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Rabbit anti-KAP-1 IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
KAP-1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 1 and 50
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Nine-banded armadillo Olive baboon Gorilla Chinese hamster White-tufted-ear marmoset Crab-eating macaque Panda Horse Small-eared galago Northern white-cheeked gibbon Thirteen-lined ground squirrel Orangutan Bovine Monkey Chimpanzee Dog Rabbit Mouse
Antigen Species
Human
Stock Concentration
100 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to KAP-1 immobilized on solid support.

The epitope recognized by IHC-00072-T maps to a region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155).

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Gene & Protein Information

KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression.

10155

TRIM28

tripartite motif containing 28

Q13263

Transcription intermediary factor 1-beta

Alternate Names

E3 SUMO-protein ligase TRIM28, KAP1, KAP-1, KRAB Kruppel-associated box domain-associated protein 1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, nuclear corepressor KAP-1, PPP1R157, protein phosphatase 1, regulatory subunit 157, RING finger protein 96, RING-type E3 ubiquitin transferase TIF1-beta, RNF96, TF1B, TIF1B, TIF1-beta, transcription intermediary factor 1-beta, transcriptional intermediary factor 1-beta, Tripartite motif-containing protein 28

Applications

Formalin-fixed, paraffin-embedded tissue 1:100 - 1:500: Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Formaldehyde-fixed cells 1:100 - 1:500: Permeabilization with Triton-X 100 is recommended. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.