Bethyl Laboratories
Rabbit anti-KIF4A Antibody Affinity Purified
Primary
Catalog #A301-073A
TargetKIF4A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
The kinesin superfamily of proteins consists of over forty KIF motor proteins that function in intracellular transport along microtubules. Kinesin activity has been linked to various cellular functions such as vesicle transport, mitotic spindle formation, chromosome segregation, and cytokinesis. Structurally, all kinesins contain a motor domain with microtubule and nucleotide binding sites that utilize ATP to target cargo along microtubule filaments. KIF14 silencing experiments suggest that KIF14 plays a multi-functional role in mitosis and cytokinesis. KIF4A has been shown to be essential for cytokinesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetKIF4A
Antigen Species
Human
Gene ID24137
Gene Symbol
KIF4A
Gene Name
kinesin family member 4A
Uniprot IDO95239
Protein Name
Chromosome-associated kinesin KIF4A
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to KIF4A immobilized on solid support.
The epitope recognized by A301-073A maps to a region between residue 1 and 50 of human kinesin family member 4A using the numbering given in entry NP_036442.3 (GeneID 24137).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-073A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-073A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-073AOpen
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