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Rabbit anti-KLHL4 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

KLHL4

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 125 and 175

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to KLHL4 immobilized on solid support.

The epitope recognized by A305-768A maps to a region between residue 125 and 175 of human Kelch-like protein 4 using the numbering given in entry Q9C0H6.2 (GeneID 56062).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to KLHL4 immobilized on solid support.

The epitope recognized by A305-768A-T maps to a region between residue 125 and 175 of human Kelch-like protein 4 using the numbering given in entry Q9C0H6.2 (GeneID 56062).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

Stromal membrane-associated protein 2 (SMAP2) is a GTPase activating protein that acts on ARF1. SMAP2 Can also activate ARF6 (in vitro) It may play a role in clathrin-dependent retrograde transport from early endosomes to the trans-Golgi network (By similarity)  [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q8WU79].

56062

KLHL4

kelch like family member 4

Q9C0H6

Kelch-like protein 4

Alternate Names

DKELCHL; kelch-like protein 4; KHL4

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.