Bethyl Laboratories
Rabbit anti-Ku70 Antibody Affinity Purified
Primary
Catalog #A302-624A
TargetKu70
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Ku70 is the 70 kDa subunit of the lupus Ku autoantigen. The lupus Ku autoantigen was identified in individuals with systemic lupus erythematosus (SLE) and related disorders. The Ku antigen is a heterodimer of Ku70 and Ku80. The Ku70/Ku80 complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P12956].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 525 and 575
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetKu70
Antigen Species
Human
Gene ID2547
Gene Symbol
XRCC6
Gene Name
X-ray repair cross complementing 6
Uniprot IDP12956
Protein Name
X-ray repair cross-complementing protein 6
Alternate Names
5'-deoxyribose-5-phosphate lyase Ku70 • 5'-dRP lyase Ku70 • 70 kDa subunit of Ku antigen • ATP-dependent DNA helicase 2 subunit 1 • ATP-dependent DNA helicase II 70 kDa subunit • ATP-dependent DNA helicase II, 70 kDa subunit • CTC box binding factor 75 kDa subunit • CTC75 • CTCBF • DNA repair protein XRCC6 • G22P1 • Ku autoantigen p70 subunit • Ku autoantigen, 70kDa • KU70 • lupus Ku autoantigen protein p70 • ML8 • thyroid autoantigen 70kDa (Ku antigen) • thyroid-lupus autoantigen p70 • TLAA • X-ray repair complementing defective repair in Chinese hamster cells 6 • X-ray repair cross-complementing protein 6
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Ku70 immobilized on solid support.
The epitope recognized by A302-624A maps to a region between residue 500 and 550 of human Lupus Ku autoantigen protein p70 using the numbering given in entry NP_001460.1 (GeneID 2547).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-624A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-624A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-624AOpen
Citations / Publications
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