Bethyl Laboratories
Rabbit anti-L3MBTL3 Antibody Affinity Purified
Primary
Catalog #A302-854A
TargetL3MBTL3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
L3MBTL3 [Lethal(3)malignant brain tumor-like protein 3] is a putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. L3MBTL3 is required for normal maturation of myeloid progenitor cells [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/9Q96JM7].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 550 and 600
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetL3MBTL3
Antigen Species
Human
Gene ID84456
Gene Symbol
L3MBTL3
Gene Name
l(3)mbt-like 3 (Drosophila)
Uniprot IDQ96JM7
Protein Name
Lethal(3)malignant brain tumor-like protein 3
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to L3MBTL3 immobilized on solid support.
The epitope recognized by A302-854A maps to a region between residue 550 and 600 of human Lethal(3)Malignant Brain Tumor-like 3 using the numbering given in entry NP_115814.1 (GeneID 84456).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-854A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-854A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-854AOpen
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