Bethyl Laboratories
Rabbit anti-LARG Antibody Affinity Purified
Primary
Catalog #A301-960A
TargetLARG
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
LARG (leukemia-associated RhoGEF) is homologous to PDZ-RhoGEF and functions as a RhoA-specific guanine nucleotide exchange factor. RhoGEFs catalyze the exchange of GDP for GTP on the GTPase, Rho, to activate downstream signaling pathways. LARG contains a PDZ [post synaptic density protein (PSD95), Drosophila discs-large tumor suppressor (DIgA), and zonula occludens-1 protein (zo-1)] domain which is an 80-90 amino acid structural domain found in signaling proteins in bacteria, yeast, plants, and animals. LARG is proposed to play a role in cell polarity and microtubule dynamics and may act as a GTPase-activating protein (GAP) for the heterotrimeric Galpha(12) and Galpha(13) proteins.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1494 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetLARG
Antigen Species
Human
Gene ID23365
Gene Symbol
ARHGEF12
Gene Name
Rho guanine nucleotide exchange factor 12
Uniprot IDQ9NZN5
Protein Name
Rho guanine nucleotide exchange factor 12
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to LARG immobilized on solid support.
The epitope recognized by A301-960A maps to a region between residue 1494 and 1544 of human leukemia-associated rho guanine nucleotide exchange factor using the numbering given in entry NP_056128.1 (GeneID 23365).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-960A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-960A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-960AOpen
Services
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