Bethyl Laboratories
Rabbit anti-LARS Antibody Affinity Purified
Primary
Catalog #A304-316A
TargetLARS
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, WB
Image 1 of 2
local_shipping
Order within 20 hours and 45 minutes for shipment by Tuesday, November 3
Product Description
Leucyl-tRNA synthetase (LARS) catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. LARS exhibits a post-transfer editing activity to hydrolyze mischarged tRNAs [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9P2J5].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetLARS
Antigen Species
Human
Gene ID51520
Gene Symbol
LARS
Gene Name
leucyl-tRNA synthetase
Uniprot IDQ9P2J5
Protein Name
Leucine--tRNA ligase, cytoplasmic
Alternate Names
cytoplasmic leucyl-tRNA synthetase • cytosolic leucyl-tRNA synthetase • hr025Cl • HSPC192 • ILFS1 • LARS1 • leucine translase • leucine tRNA ligase 1, cytoplasmic • leucine--tRNA ligase, cytoplasmic • Leucyl-tRNA synthetase • LEURS • LEUS • LFIS • LRS • PIG44 • proliferation-inducing gene 44 • RNTLS
Product Information
Applications
IHC, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to LARS immobilized on solid support.
The epitope recognized by A304-316A maps to a region between residue 250 to 300 of human Leucyl-tRNA Synthetase using the numbering given in entry NP_064502.9 (GeneID 51520).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000 - 1:5000
Documents
Product DatasheetPDF · A304-316A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-316A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-316AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1