Bethyl Laboratories
Rabbit anti-Lasu1/Ureb1 Antibody Affinity Purified
Primary
Catalog #A300-486A
TargetLasu1/Ureb1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Lasu1/Ureb1 is an E3 ubiquitin protein ligase that mediates the ubiquitination of core histones, Mcl-1, Cdc6, and p53. Lasu1/Ureb1 expression is regulated during spermatogenesis and appears to play a role in chromatin modification in germ cells as well as other tissues.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2275 and 2325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetLasu1/Ureb1
Antigen Species
Human
Gene ID10075
Gene Symbol
HUWE1
Gene Name
HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase
Uniprot IDQ7Z6Z7
Protein Name
E3 ubiquitin-protein ligase HUWE1
Alternate Names
ARF-binding protein 1 • ARF-BP1 • BJ-HCC-24 tumor antigen • E3 ubiquitin-protein ligase HUWE1 • HECT domain protein LASU1 • HECT, UBA and WWE domain-containing protein 1 • HECTH9 • HECT-type E3 ubiquitin transferase HUWE1 • homologous to E6AP carboxyl terminus homologous protein 9 • HSPC272 • Ib772 • large structure of UREB1 • LASU1 • Mcl-1 ubiquitin ligase E3 • MULE • upstream regulatory element-binding protein 1 • UREB1 • URE-B1 • URE-binding protein 1
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Ureb1/Lasu1 immobilized on solid support.
The epitope recognized by A300-486A maps to a region between residues 2250 and 2300 of human Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.3 (GeneID 10075).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:1000 to 1:5000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A300-486A · Lot 10 (current lot)Open
Product DatasheetPDF · A300-486A · Lot 10 (current lot)Open
Safety Data SheetPDF · A300-486AOpen
Citations / Publications
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