Bethyl Laboratories
Rabbit anti-LGN Antibody Affinity Purified
Primary
Catalog #A303-031A
TargetLGN
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 9 hours and 15 minutes for shipment by today
Product Description
LGN (Leu-Gly-Asn repeat-enriched protein) plays an important role in spindle pole orientation. LGN interacts and contributes to the functional activity of G(i) alpha proteins. LGN acts to stabilize the apical complex during neuroblast divisions.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 450 and 500
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetLGN
Antigen Species
Human
Gene ID29899
Gene Symbol
GPSM2
Gene Name
G-protein signaling modulator 2
Uniprot IDP81274
Protein Name
G-protein-signaling modulator 2
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to LGN immobilized on solid support.
The epitope recognized by A303-031A maps to a region between residue 450 and 500 of human Mosaic Protein LGN using the numbering given in entry AAH27732.1 (GeneID 29899).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-031A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-031A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-031AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1