Bethyl Laboratories
Rabbit anti-LIMK1 Antibody Affinity Purified
Primary
Catalog #A302-670A
TargetLIMK1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
LIM domain kinase 1 (LIMK1) is a serine/threonine protein kinase that bears 2 LIM zinc-binding domains. LIMK1 participates in the regulation of actin filament dynamics and may play a role in synapse formation and function.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 275 and 325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetLIMK1
Antigen Species
Human
Gene ID3984
Gene Symbol
LIMK1
Gene Name
LIM domain kinase 1
Uniprot IDP53667
Protein Name
LIM domain kinase 1
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to LIMK1 immobilized on solid support.
The epitope recognized by A302-670A maps to a region between residue 250 and 300 of human LIM domain kinase 1 using the numbering given in entry NP_002305.1 (GeneID 3984).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-670A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-670A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-670AOpen
Services
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