/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-LIN28B Antibody
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Primary Antibodies  

Rabbit anti-LIN28B Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

LIN28B

Reactivity:

Human

Applications:

IHC

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 200 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Country of Origin

USA

Additional Product Information

Lin-28 homolog B (LIN28B) acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. LIN28B acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. LIN28B specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. It also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q6ZN17].

389421

LIN28B

lin-28 homolog B

Q6ZN17

Protein lin-28 homolog B

Alternate Names

CSDD2; Lin-28.2; lin-28B; protein lin-28 homolog B

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.