Bethyl Laboratories
Rabbit anti-LIN54 Antibody Affinity Purified
Primary
Catalog #A303-799A
TargetLIN54
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
LIN54 is a component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, LIN54 acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6MZP7].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetLIN54
Antigen Species
Human
Gene ID132660
Gene Symbol
LIN54
Gene Name
lin-54 DREAM MuvB core complex component
Uniprot IDQ6MZP7
Protein Name
Protein lin-54 homolog
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to LIN54 immobilized on solid support.
The epitope recognized by A303-799A maps to a region between residue 1 and 50 of human Lin-54 homolog using the numbering given in entry NP_919258.2 (GeneID 132660).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-799A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-799A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-799AOpen
Citations / Publications
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