Bethyl Laboratories
Rabbit anti-LRPPRC Antibody Affinity Purified
Primary
Catalog #A304-731A
TargetLRPPRC
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
LRPPRC is a leucine-rich protein that has multiple pentatricopeptide repeats (PPR). The precise role of this protein is unknown but studies suggest it may play a role in cytoskeletal organization, vesicular transport, or in transcriptional regulation of both nuclear and mitochondrial genes. The protein localizes primarily to mitochondria and is predicted to have an N-terminal mitochondrial targeting sequence. Mutations in this gene are associated with the French-Canadian type of Leigh syndrome [taken from NCBI Entrez Gene (Gene ID: 10128)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1344 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetLRPPRC
Antigen Species
Human
Gene ID10128
Gene Symbol
LRPPRC
Gene Name
leucine rich pentatricopeptide repeat containing
Uniprot IDP42704
Protein Name
Leucine-rich PPR motif-containing protein, mitochondrial
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to LRPPRC immobilized on solid support.
The epitope recognized by A304-731A maps to a region between residue 1344 to 1394 of human Leucine-Rich Pentatricopeptide Repeat Containing using the numbering given in entry NP_573566.2 (GeneID 10128).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A304-731A · Lot 1 (current lot)Open
Product DatasheetPDF · A304-731A · Lot 1 (current lot)Open
Safety Data SheetPDF · A304-731AOpen
Citations / Publications
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