Bethyl Laboratories
Rabbit anti-LYRIC/AEG1 Recombinant Monoclonal Antibody [BLR258L]
Primary
Catalog #A700-258
TargetLYRIC/AEG1
HostRabbit
CloneBLR258L
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
Astrocyte elevated gene-1 protein (AEG1) is also known as metadherin. AEG1 AEG1 promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. It also promotes lung metastasis and has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. AEG1 induces chemoresistance.
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR258L
Host
Rabbit
Immunogen
Between 475 and 525
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
250 µg/ml
Target Identity
TargetLYRIC/AEG1
Antigen Species
Human
Gene ID92140
Gene Symbol
MTDH
Gene Name
metadherin
Uniprot IDQ86UE4
Protein Name
Protein LYRIC
Alternate Names
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 450 and 500 of human protein LYRIC using the numbering given in entry NP_848927.2 (Gene ID 92140).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:50 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:50 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-258 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-258 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-258 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-258Open
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Resources
Standard Western Blot Protocol
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Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
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