Bethyl Laboratories
Rabbit anti-MAD2 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00412
TargetMAD2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
MAD2 (Mitotic Arrest Deficiency 2) is a component of the mitotic spindle-assembly checkpoint that ensures that all chromosomes are aligned at the metaphase plate before the onset of anaphase. MAD2 is a subunit of the MCC complex that includes BubR1, Bub3, Cdc20 and Mad2, which may function to inhibit the APC/c (Anaphase Promoting Complex/Cyclosome). MAD2 is regulated by MAD1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 100 and 150
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetMAD2
Antigen Species
Human
Gene ID4085
Gene Symbol
MAD2L1
Gene Name
mitotic arrest deficient 2 like 1
Uniprot IDQ13257
Protein Name
Mitotic spindle assembly checkpoint protein MAD2A
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MAD2 immobilized on solid support.
The epitope recognized by IHC-00412 maps to a region between residues 100 and 150 of human Mitotic Arrest Deficient 2 using the numbering given in SwissProt entry Q13257 (GeneID 4085).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00412Open
Product DatasheetPDF · IHC-00412 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00412 · Lot 1 (current lot)Open
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