Bethyl Laboratories

Rabbit anti-MafA Antibody Affinity Purified

Primary
Catalog #A300-611A
TargetMafA
HostRabbit
ClonalityPolyclonal
ReactivityMouse
ApplicationWB
Detection of MafA, MafB and cMaf by western blot.
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Product Description

MafA is a member of the Maf basic leucine-zipper containing transcription factor family. MafA is an islet-enriched protein that plays a role in beta cell-specific and glucose-responsive insulin gene transcription. MafA is expressed exclusively in cell populations that will become islet beta cells.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 309 and C-terminus
Isotype
IgG
Reactivity
Mouse
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMafA
Antigen Species
Mouse
Gene ID378435
Gene Symbol
Mafa
Gene Name
v-maf musculoaponeurotic fibrosarcoma oncogene family, protein A (avian)
Uniprot IDQ8CF90
Protein Name
Transcription factor MafA
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

The epitope recognized by A300-611A maps to a region between residue 300 and the C-terminus (residue 359) of mouse v-maf musculoaponeurotic fibrosarcoma oncogene homolog A using the numbering given in entry NP_919331. 1 (GeneID 378435).

Antibody was affinity purified using the peptide immobilized on solid support.

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).

Western Blot (WB)1:1000 - 1:10,000

Documents

Product DatasheetPDF · A300-611A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-611A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-611AOpen

Citations / Publications

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