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Rabbit anti-MAML1 IHC Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
MAML1
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 75 and 125
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (50+ slides)

10 µl (5+ slides)

For ordering information, see our International Distributors

Product Details

Specifications
Verified Reactivity
Human
Presumed Reactivity
Chimpanzee
Antigen Species
Human
Stock Concentration
250 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to MAML1 immobilized on solid support.

The epitope recognized by IHC-00445-T maps to a region between residue 75 and 125 of human Mastermind-like 1 using the numbering given in entry NP_055572.1 (GeneID 9794).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

There are 3 MAML (mastermind-like) proteins in mammals (MAML1-3). MAML1 is a critical coactivator for NOTCH signaling. MAML1 associates with NOTCH and CSL in a ternary complex and functions to activate transcription of HES and hairy-related basic helix-loop-helix transcription factors. MAML1 has been observed to interact with p300 to acetylate histones H3 and H4. MAML1 also interacts with p53 and functions independent of NOTCH signaling, as a coactivator of p53 target genes.

9794

MAML1

mastermind like transcriptional coactivator 1

Q92585

Mastermind-like protein 1

Alternate Names

Mam1, Mam-1, mastermind homolog, mastermind-like 1, mastermind-like protein 1

Applications

Epitope exposure is recommended. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated.