Bethyl Laboratories
Rabbit anti-MAML2 Antibody Affinity Purified
Primary
Catalog #A300-682A
TargetMAML2
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
There are 3 MAML (mastermind-like) proteins in mammals (MAML1-3). MAML2 is a critical coactivator for NOTCH signaling. MAML2 associates with NOTCH and CSL in a ternary complex and functions to activate transcription of HES and hairy-related basic helix-loop-helix transcription factors. MAML2 has been identified in a chromosomal rearrangement and fusion with MECT1 in mucoepidermoid carcinomas, secondary acute myeloid leukemia, hidradenomas, and myelodysplastic syndrome. MECT1 is a CREB regulator, and when fused with the transactivational domain of MAML2, imparts constitutive activation of CREB-dependent transcription that may promote oncogenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1106 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetMAML2
Antigen Species
Human
Gene ID84441
Gene Symbol
MAML2
Gene Name
mastermind like transcriptional coactivator 2
Uniprot IDQ8IZL2
Protein Name
Mastermind-like protein 2
Alternate Names
MAM2 • mam-2 • MAM3 • MAM-3 • mastermind-like 2 • mastermind-like protein 2 • MLL-MAML2
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MAML2 immobilized on solid support.
The epitope recognized by A300-682A maps to a region between residue 1100 and the C-terminus (residue 1153) of human Mastermind-Like 2 using the numbering given in entry AAK93831.1 (GeneID 84441).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:15,000
Documents
Product DatasheetPDF · A300-682A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-682A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-682AOpen
Citations / Publications
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