Bethyl Laboratories
Rabbit anti-MAML2 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00446
TargetMAML2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
There are 3 MAML (mastermind-like) proteins in mammals (MAML1-3). MAML2 is a critical coactivator for NOTCH signaling. MAML2 associates with NOTCH and CSL in a ternary complex and functions to activate transcription of HES and hairy-related basic helix-loop-helix transcription factors. MAML2 has been identified in a chromosomal rearrangement and fusion with MECT1 in mucoepidermoid carcinomas, secondary acute myeloid leukemia, hidradenomas, and myelodysplastic syndrome. MECT1 is a CREB regulator, and when fused with the transactivational domain of MAML2, imparts constitutive activation of CREB-dependent transcription that may promote oncogenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 275 and 325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetMAML2
Antigen Species
Human
Gene ID84441
Gene Symbol
MAML2
Gene Name
mastermind like transcriptional coactivator 2
Uniprot IDQ8IZL2
Protein Name
Mastermind-like protein 2
Alternate Names
MAM2 • mam-2 • MAM3 • MAM-3 • mastermind-like 2 • mastermind-like protein 2 • MLL-MAML2
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MAML2 immobilized on solid support.
The epitope recognized by IHC-00446 maps to a region between residue 1100 and the C-terminus (residue 1153) of human Mastermind-Like 2 using the numbering given in entry AAK93831.1 (GeneID 84441).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00446Open
Product DatasheetPDF · IHC-00446 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00446 · Lot 1 (current lot)Open
Citations / Publications
Services
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