/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-MAP1B Antibody
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Primary Antibodies  

Rabbit anti-MAP1B Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

MAP1B

Reactivity:

Human

Applications:

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 2050 and 2100

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to MAP1B immobilized on solid support.

The epitope recognized by A301-447A-T maps to a region between residue 2050 and 2100 of human microtubule-associated protein 1B using the numbering given in entry NP_005900.2 (GeneID 4131).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to MAP1B immobilized on solid support.

The epitope recognized by A301-447A maps to a region between residue 2050 and 2100 of human microtubule-associated protein 1B using the numbering given in entry NP_005900.2 (GeneID 4131).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

MAP1B is one of the founding members of the MAP (microtubule-associated protein) family of proteins involved in microtubule assembly. MAP1B is almost exclusively expressed in the developing brain and is critical for axon formation. MAP1B-null mice exhibit an absence of the corpus collosum and impaired axon development. MAP1B is processed by proteolytic cleavage that leads to the generation of a 300 kDa heavy chain and a 32 kDa LC1 light chain.

4131

MAP1B

microtubule associated protein 1B

P46821

Microtubule-associated protein 1B

Alternate Names

FUTSCH; MAP-1B; MAP5; microtubule-associated protein 1B; PPP1R102; protein phosphatase 1, regulatory subunit 102

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.