Bethyl Laboratories
Rabbit anti-MAP1B Antibody Affinity Purified
Primary
Catalog #A301-447A
TargetMAP1B
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
MAP1B is one of the founding members of the MAP (microtubule-associated protein) family of proteins involved in microtubule assembly. MAP1B is almost exclusively expressed in the developing brain and is critical for axon formation. MAP1B-null mice exhibit an absence of the corpus collosum and impaired axon development. MAP1B is processed by proteolytic cleavage that leads to the generation of a 300 kDa heavy chain and a 32 kDa LC1 light chain.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2050 and 2100
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMAP1B
Antigen Species
Human
Gene ID4131
Gene Symbol
MAP1B
Gene Name
microtubule associated protein 1B
Uniprot IDP46821
Protein Name
Microtubule-associated protein 1B
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MAP1B immobilized on solid support.
The epitope recognized by A301-447A maps to a region between residue 2050 and 2100 of human microtubule-associated protein 1B using the numbering given in entry NP_005900.2 (GeneID 4131).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-447A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-447A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-447AOpen
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