Bethyl Laboratories
Rabbit anti-MAP4K4/HGK Antibody Affinity Purified
Primary
Catalog #A301-503A
TargetMAP4K4/HGK
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
MAP4K4 is a serine/threonine kinase that signals upstream of the c-Jun N-terminal kinase (JNK) and is involved in cell motility. MAP4K4 appears to partially mediate TNF alpha signaling. In skeletal muscle TNF-alpha induces insulin resistance. TNF-alpha induced insulin resistance has been shown to be dependent on MAPK4K4 activity. Interestingly, TNF-alpha regulates MAP4K4 expression and can stimulate MAP4K4 gene expression via TNFR2 (TNFalpha receptor 2) and the c-Jun and ATF2 (activating transcription factor 2) transcription factors. MAP4K4 is highly expressed in most tumor cell lines, and the analysis of MAP4K4 mutants indicates that it may have an important role in cell transformation and invasiveness.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 875 and 925
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMAP4K4/HGK
Antigen Species
Human
Gene ID9448
Gene Symbol
MAP4K4
Gene Name
mitogen-activated protein kinase kinase kinase kinase 4
Uniprot IDO95819
Protein Name
Mitogen-activated protein kinase kinase kinase kinase 4
Alternate Names
epididymis secretory protein Li 31 • FLH21957 • HEL-S-31 • hepatocyte progenitor kinase-like/germinal center kinase-like kinase • HGK • HPK/GCK-like kinase HGK • MAPK/ERK kinase kinase kinase 4 • MEK kinase kinase 4 • MEKKK 4 • MEKKK4 • mitogen-activated protein kinase kinase kinase kinase 4 • nck-interacting kinase • NIK • Ste20 group protein kinase HGK
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MAP4K4/HGK immobilized on solid support.
The epitope recognized by A301-503A maps to a region between residue 850 and 900 of human MAPK/ERK kinase kinase kinase 4 (hepatocyte progenitor kinase-like/germinal center kinase-like kinase) using the numbering given in entry AAO32626.1 (GeneID 9448).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-503A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-503A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-503AOpen
Citations / Publications
Services
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