Bethyl Laboratories
Rabbit anti-MAP4K4/HGK IHC Antibody Affinity Purified
Primary
Catalog #IHC-00536
TargetMAP4K4/HGK
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
MAP4K4 is a serine/threonine kinase that signals upstream of the c-Jun N-terminal kinase (JNK) and is involved in cell motility. MAP4K4 appears to partially mediate TNF alpha signaling. In skeletal muscle TNF-alpha induces insulin resistance. TNF-alpha induced insulin resistance has been shown to be dependent on MAPK4K4 activity. Interestingly, TNF-alpha regulates MAP4K4 expression and can stimulate MAP4K4 gene expression via TNFR2 (TNFalpha receptor 2) and the c-Jun and ATF2 (activating transcription factor 2) transcription factors. MAP4K4 is highly expressed in most tumor cell lines, and the analysis of MAP4K4 mutants indicates that it may have an important role in cell transformation and invasiveness.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 550 and 600
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetMAP4K4/HGK
Antigen Species
Human
Gene ID9448
Gene Symbol
MAP4K4
Gene Name
mitogen-activated protein kinase kinase kinase kinase 4
Uniprot IDO95819
Protein Name
Mitogen-activated protein kinase kinase kinase kinase 4
Alternate Names
epididymis secretory protein Li 31 • FLH21957 • HEL-S-31 • hepatocyte progenitor kinase-like/germinal center kinase-like kinase • HGK • HPK/GCK-like kinase HGK • MAPK/ERK kinase kinase kinase 4 • MEK kinase kinase 4 • MEKKK 4 • MEKKK4 • mitogen-activated protein kinase kinase kinase kinase 4 • nck-interacting kinase • NIK • Ste20 group protein kinase HGK
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MAP4K4/HGK immobilized on solid support.
The epitope recognized by IHC-00536 maps to a region between residue 550 and 600 of human MAPK/ERK kinase kinase kinase 4 (hepatocyte progenitor kinase-like/germinal center kinase-like kinase) using the numbering given in entry AAO32626.1 (GeneID 9448).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00536Open
Product DatasheetPDF · IHC-00536 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00536 · Lot 1 (current lot)Open
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