Bethyl Laboratories
Rabbit anti-MAP7 Antibody Affinity Purified
Primary
Catalog #A301-491A
TargetMAP7
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
Image 1 of 2
local_shipping
Order within 11 hours and 12 minutes for shipment by Tuesday, November 17
Product Description
MAP7 was first identified in HeLa cells as E-MAP-115. It is predominantly expressed in cells of epithelial origin and proposed to function as a stabilizer of microtubules important for regulating cell polarization. Disruption of the MAP7 gene has been associated with cases of sacral dysgenesis; however MAP7 knockout mice do not display the associated phenotype. In mice it has been shown to be important to sperm cell development.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 699 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetMAP7
Antigen Species
Human
Gene ID9053
Gene Symbol
MAP7
Gene Name
microtubule associated protein 7
Uniprot IDQ14244
Protein Name
Ensconsin
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MAP7 immobilized on solid support.
The epitope recognized by A301-491A maps to a region between residue 720 and 749 of human microtubule-associated protein 7 using the numbering given in entry NP_003971.1 (GeneID 9053).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-491A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-491A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-491AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1