Bethyl Laboratories
Rabbit anti-Matriptase/ST14 Antibody Affinity Purified
Primary
Catalog #A300-221A
TargetMatriptase/ST14
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Matriptase/ST14 is an epithelial-derived type II transmembrane trypsin-like serine protease that has been shown to cleave and activate various proteases and growth factors. The overexpression of Matriptase /ST14 in a variety of tumors, and its ability to degrade extracellular matrix suggests a role in tumor invasion.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 805 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMatriptase/ST14
Antigen Species
Human
Gene ID6768
Gene Symbol
ST14
Gene Name
suppression of tumorigenicity 14
Uniprot IDQ9Y5Y6
Protein Name
Suppressor of tumorigenicity 14 protein
Alternate Names
ARCI11 • HAI • Matriptase • membrane-type serine protease 1 • MTSP1 • MT-SP1 • prostamin • PRSS14 • serine protease 14 • serine protease TADG-15 • SNC19 • suppression of tumorigenicity 14 (colon carcinoma) • suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin) • suppressor of tumorigenicity 14 protein • TADG15 • TMPRSS14 • tumor associated differentially expressed gene 15 protein • Tumor-associated differentially-expressed gene 15 protein
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Matriptase/ST14 immobilized on solid support.
The epitope recognized by A300-221A maps to a region between residues 800 and the C-terminus (residue 855) of human Membrane-type serine protease 1 using the numbering given in SwissProt entry Q9Y5Y6 (GeneID 6768).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:10,000 - 1:25,000
Documents
Product DatasheetPDF · A300-221A · Lot 3 (current lot)Open
Product DatasheetPDF · A300-221A · Lot 3 (current lot)Open
Safety Data SheetPDF · A300-221AOpen
Citations / Publications
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