Bethyl Laboratories
Rabbit anti-MAX Antibody Affinity Purified
Primary
Catalog #A302-866A
TargetMAX
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
MAX (Myc-associated factor X) is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation [taken from NCBI Entrez Gene (Gene ID: 4149)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 110 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetMAX
Antigen Species
Human
Gene ID4149
Gene Symbol
MAX
Gene Name
MYC associated factor X
Uniprot IDP61244
Protein Name
Protein max
Alternate Names
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to MAX immobilized on solid support.
The epitope recognized by A302-866A maps to a region between residue 110 and 160 of human MYC Associated Factor X using the numbering given in entry NP_002373.3 (GeneID 4149).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-866A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-866A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-866AOpen
Citations / Publications
Services
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